Our long term experience with QFPCR verified the protocol of Pertl et al. [9], with slight modifications de­scribed in detail, including interpretation problems (34). The analyses were performed on an ABI PRISM 310 (Ap­plied BioSystems, Foster City, CA, USA). The panel of primers for the detection of aneuploidies of chromosomes 13, 18 and 21 was extended by us as well as for X and Y aneuploidy detection according to Cirigliano et al. [24,25,36] [Table 1(a)]. New STR markers for hetero-chromosomes that increase the genetic informativity and detection of their aneuploidy are documented on Table 1(b).