Balkan Journal of Medical Genetics

EVALUATION OF METHYLATION PROFILES OF AN EPIDERMAL GROWTH FACTOR RECEPTOR GENE IN A HEAD AND NECK SQUAMOUS CELL CARCINOMA PATIENT GROUP
Mutlu M, Mutlu P, Azarkan S, Bayır O, Ocal B, Saylam G, Korkmaz MH
*Corresponding Author: Associate Professor Murad Mutlu, M.D., Department of Otorhinolaringology, Health Sciences University, Ministry of Health, Dışkapı Yildirim Beyazit Training and Research Hopsital, Sehit Ömer Halisdemir Street, No. 20, 06110, Dışkapı, Ankara, Turkey. Tel.: +90-312-516-2000. Fax: +90-312-318-6690. E-mail: muradmutlu78@yahoo.com
page: 65

RESULTS

Clinicopathological Parameters of Control and Patient Groups. Control group were selected to match the patients in terms of demographic data including age and gender. The median ages of control and patient groups were 58 and 59, respectively. On the other hand, 13.0% of the patients were female and 87.0% were male, whereas 25.0% of the healthy individuals were female and 75.0% were male. The most common tumor originated on the larynx (65.96%), followed by hypopharynx (10.64%) among the HNSCC patient group in our study. According to pathological staging, 21.27% patients were reported as being in T1, 21.27% in T2, 42.55% in T3 and 14.89% in T4 stages (Table 1). Methylation Profile. Promoter methylation of the EGFR gene was detected by MS-PCR following bisulfite modification of DNAs (Figure 1 and Figure 2). Cycle threshold values obtained from both healthy individuals and patients were compared with the Ct values of control DNA, and the methylation profiles were determined for each individual. If the methylated reaction is positive and the unmethylated reaction is negative or both reactions are positive, the methylation status is evaluated as methylated. On the other hand, if the methylated reaction is negative and the unmethylated reaction is positive, methylation status is determined as unmethylated. Samples with negative reaction of both methylated and unmethylated were excluded from the study (Table 3 and Table 4). According to the MS-PCR results, methylation was observed in 79.0% of patients, whereas methylation was not observed in 21.0% of patients. On the other hand, in the control group, methylation was observed in 90.0% healthy individuals (Table 5). The methylation profile of the EGFR gene promoter was also compared with age, gender, and clinicopathological characteristics in the control and patient groups (Table 6). There is no statistically significant difference in terms of age, gender and clinicopathological characteristics.

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