Balkan Journal of Medical Genetics

MOLECULAR CYTOGENETIC STUDY OF THE NF2 GENE DELETION IN MENINGIOMA IN SUDANESE PATIENTS
AbdElmontalab Farah Y1, Fadl Elmula I2, Abushama HM1, Kreskowski K3, Liehr T3,*
*Corresponding Author: Dr. Thomas Liehr, Institut für Humangenetik, Kollegiengasse 10, D-07740 Jena, Germany; Tel.: 49-3641-935533; Fax: ++49-3641-935582; E-mail: Thomas.Liehr@med.uni-jena.de
page: 29

MATERIALS AND METHODS

Tumor Samples and Cultivation. This study was approved by the Ethical Review Board at Neilein University, Khartoum, Sudan and informed consent was obtained from all patients. Cross-sectional design was used in this study. Twentynine meningioma tissue samples (Table 1) were collected during surgeries from the Alshaab Teaching Hospital located in Khartoum State, Sudan. Thirteen patients were male (44.8%) and 16 female (55.2%), aged 15 to 55 years, thus being much younger than meningioma patients in Western countries. Samples were collected in sterile containers with sterile RBMI-1640 media to be processed for tissue culture within two hours. Cytogenetic and Molecular Cytogenetics. After long-term culture, chromosomes were prepared and GTG-banding was done using standard procedures [6]. Interphase fluorescence in situ hybridization (I-FISH) was performed using a two-color FISH approach: DNA derived from BAC-probe RP11-551L12 was NF2 gene-specific (22q12.2) and labeled with Texas Red; RP11-172D7-DNA located in 22q11.21 served as an internal control and was labeled in SpectrumGreen. The FISH-procedure was done according to standard protocols [7]. For microscopic evaluation, 100 interphase nuclei were examined for each specimen.

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