Table 1. Frequency of Y chromosome microdeletions among unselected infertile males from the Republic of Macedonia

Table 2. Andrological findings in infertile males with Y chromosome microdeletions

Normal values:            MTV, 18.25+6.27;  FSH, 1.6-12;  LH, 0.8-6.0;  Testosterone, 5.2-22.9
    * varicocele

The andrological findings of the patients with Y-chromosome microdeletions are given in Table 2. The testis volume, hormonal status and histological findings were available from three patients with AZFc deletion. Two of them (patients I.V and S.B.) presented with reduced testicular volumes, increased FSH, normal LH and testosterone values, azoospermia and testicular histology of SCOS. In addition, varicocele of the left testis was detected in patient S.B. Patient A.A. presented with severe oligozoospermia and hypospermatogenesis; his testicular volumes and hormonal values were in the normal range.
The father of only one patient was available for analysis, and he did not carry the deletion. The paternity was confirmed by DNA analysis.

Figure 1. Agarose gel electrophoresis showing the separation of the PCR fragments obtained by Multiplex A and Multiplex B PCR reactions. Line 1, DNA of infertile male with AZFb+c deletion; Line 2, DNA of infertile male without AZF deletions; Lines 3, DNA of infertile male with AZFc deletion; Line 4, control (fertile) male DNA; Line 5, female DNA; Line 6, Blank (no DNA).