Balkan Journal of Medical Genetics

A NOVEL c.973G>T MUTATION IN THE ε-SUBUNIT OF THE ACETYLCHOLINE RECEPTOR CAUSING CONGENITAL MYASTHENIC SYNDROME IN AN IRANIAN FAMILY
Karimzadeh P1,2, Parvizi Omran S3, Ghaedi H4, Omrani MD4,*
*Corresponding Author: Mir Davood Omrani, Ph.D., Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Koodakyar Street, Daneshjoo Boulevard, Evin, Chamran Highway, Tehran, Islamic Republic of Iran, 1985717443. E-mail: davood_omrani@sbmu.ac.ir
page: 95

RESULTS

Mutation Analysis. Direct sequencing of all 12 exons of the CHRNE gene, including all intron-exon boundaries, revealed the plausible presence of a novel missense mutation in a homozygous state. The mutation was found in exon 11, and substitution of the nucleotide G>T at the position 973 of the cDNA (c.973G>T) [Figure 2(a)]. This results in a substitution of valine to leucine amino acid at position 325 (V325L) of the protein. In addition, the target sequencing of family members revealed that both parents carried the same mutation in the heterozygous state [Figure 2(b)]. This formally confirmed that this patient likely inherited the mutation from each parent. No homozygous or heterozygous mutations were detected in the patient’s siblings. Pathogenicity of a Novel Mutation. We predicted the functional consequences of the newly identified mutation in the CHRNE gene through three in silico prediction tools. Here, the PolyPhen 2 result revealed that the c.973 G>T mutation probably damaged the structure and function of protein with a score of 0.999. According to the MutationTaster, the V325L mutation was predicted to be the causal agent for disease. Moreover, SIFT analysis showed the mutation is deleterious with the score of 0.01. The variant was not annotated in the National Center for Biotechnology Information (NCBI), database for single nucleotide polymorphism (dbSNP) [8], 1000 Genomes Project [9] Exome Variant Server (http://evs.gs.washing ton.edu/EVS) and was also not listed in ClinVar (http:// www.ncbi.nlm.nih.gov/clinvar/). Furthermore, the identified variant was recognized as “likely pathogenic” according to ACMG/AMP recommendations [10].

Number 27 VOL. 27 (2), 2024	Number 27 VOL. 27 (1), 2024
Number 26 Number 26 VOL. 26(2), 2023 All in one	Number 26 VOL. 26(2), 2023
Number 26 VOL. 26, 2023 Supplement	Number 26 VOL. 26(1), 2023
Number 25 VOL. 25(2), 2022	Number 25 VOL. 25 (1), 2022
Number 24 VOL. 24(2), 2021	Number 24 VOL. 24(1), 2021
Number 23 VOL. 23(2), 2020	Number 22 VOL. 22(2), 2019
Number 22 VOL. 22(1), 2019	Number 22 VOL. 22, 2019 Supplement
Number 21 VOL. 21(2), 2018	Number 21 VOL. 21 (1), 2018
Number 21 VOL. 21, 2018 Supplement	Number 20 VOL. 20 (2), 2017
Number 20 VOL. 20 (1), 2017	Number 19 VOL. 19 (2), 2016
Number 19 VOL. 19 (1), 2016	Number 18 VOL. 18 (2), 2015
Number 18 VOL. 18 (1), 2015	Number 17 VOL. 17 (2), 2014
Number 17 VOL. 17 (1), 2014	Number 16 VOL. 16 (2), 2013
Number 16 VOL. 16 (1), 2013	Number 15 VOL. 15 (2), 2012
Number 15 VOL. 15, 2012 Supplement	Number 15 Vol. 15 (1), 2012
Number 14 14 - Vol. 14 (2), 2011	Number 14 The 9th Balkan Congress of Medical Genetics
Number 14 14 - Vol. 14 (1), 2011	Number 13 Vol. 13 (2), 2010
Number 13 Vol.13 (1), 2010	Number 12 Vol.12 (2), 2009
Number 12 Vol.12 (1), 2009	Number 11 Vol.11 (2),2008
Number 11 Vol.11 (1),2008	Number 10 Vol.10 (2), 2007
Number 10 10 (1),2007	Number 9 1&2, 2006
Number 9 3&4, 2006	Number 8 1&2, 2005
Number 8 3&4, 2004	Number 7 1&2, 2004
Number 6 3&4, 2003	Number 6 1&2, 2003
Number 5 3&4, 2002	Number 5 1&2, 2002
Number 4 Vol.3 (4), 2000	Number 4 Vol.2 (4), 1999
Number 4 Vol.1 (4), 1998	Number 4 3&4, 2001
Number 4 1&2, 2001	Number 3 Vol.3 (3), 2000
Number 3 Vol.2 (3), 1999	Number 3 Vol.1 (3), 1998
Number 2 Vol.3(2), 2000	Number 2 Vol.1 (2), 1998
Number 2 Vol.2 (2), 1999	Number 1 Vol.3 (1), 2000
Number 1 Vol.2 (1), 1999	Number 1 Vol.1 (1), 1998

About the journal ::: Editorial ::: Subscription ::: Information for authors ::: Contact