Balkan Journal of Medical Genetics

FREQUENCY AND ASSOCIATION OF 1691 (G>A) FVL, 20210 (G>A) PT AND 677 (C>T) MTHFR WITH DEEP VEIN THROMBOSIS IN THE POPULATION OF BOSNIA AND HERZEGOVINA
Jusić-Karić A, Terzić R, Jerkić Z, Avdić A, Pođanin M
*Corresponding Author: Amela Jusić-Karić, Ph.D., Faculty of Science and Mathematics, University of Tuzla, Univerzitetska 4, 75 000 Tuzla, Bosnia and Herzegovina. Tel: +387-61-289-217. Fax: +387-35-320-861. E-mail: amela.jusic @untz.ba
page: 43

MATERIALS AND METHODS

Subjects. Our study included 111 patients with DVT (52 males, 59 females). The median age of patients was 53 years (ranging from 21 to 84). Two hundred and seven healthy individuals were included in the control group (102 males, 105 females), their median age was 45 years (ranging from 18 to 84), without a history of known risk factors for DVT. The samples were collected at the Clinics for Cardiovascular Diseases at the University Clinical Centre Tuzla, Tuzla, Bosnia and Herzegovina. Our investigation was carried out in the Laboratory of Molecular Medicine at the Clinic for Laboratory Diagnostics, University Clinical Centre Tuzla. All cases and controls were fully informed about the study protocol by the main investigator and agreed to participate in the study by signing the written consent form. The study was approved by the Ethics Committee of the University Clinica Centre Tuzla (decision reference numbers 01/1-37-4-25/11 and 01/3-37-25/11). Methods. The DNA was isolated from EDTA anti-coagulated whole blood (Vacutainer Becton Dickinson, Meylan Cedex, France) using the commercial FlexiGene DNA Isolaton Kit (250) (Qiagen GmbH, Hilden, Ger- many). The 1691, 20210 and 677 mutations were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques that were in accordance with previously described protocols [8,14,15]. The genotypes were determined by electrophoresis in 4.0% agarose gel (Sigma Aldrich Chemie GmbH, Münich, Germany) stained with ethidium bromide (Sigma Aldrich). Statistical Analyses. Statistical analyses were performed by using MedCalc 12.4.0.0 (MedCalc Software, Ostend, Belgium). Deviation of allele and genotype distribution from the Hardy-Weinberg equilibrium was assessed by the χ2 test. For all mutations, the odds ratio (OR) and their 95% confidence intervals (CI) were calculated according to McHugh [16] to estimate the risk for DVT. Statistical significance was set at a p value of <0.05.

Number 27 VOL. 27 (2), 2024	Number 27 VOL. 27 (1), 2024
Number 26 Number 26 VOL. 26(2), 2023 All in one	Number 26 VOL. 26(2), 2023
Number 26 VOL. 26, 2023 Supplement	Number 26 VOL. 26(1), 2023
Number 25 VOL. 25(2), 2022	Number 25 VOL. 25 (1), 2022
Number 24 VOL. 24(2), 2021	Number 24 VOL. 24(1), 2021
Number 23 VOL. 23(2), 2020	Number 22 VOL. 22(2), 2019
Number 22 VOL. 22(1), 2019	Number 22 VOL. 22, 2019 Supplement
Number 21 VOL. 21(2), 2018	Number 21 VOL. 21 (1), 2018
Number 21 VOL. 21, 2018 Supplement	Number 20 VOL. 20 (2), 2017
Number 20 VOL. 20 (1), 2017	Number 19 VOL. 19 (2), 2016
Number 19 VOL. 19 (1), 2016	Number 18 VOL. 18 (2), 2015
Number 18 VOL. 18 (1), 2015	Number 17 VOL. 17 (2), 2014
Number 17 VOL. 17 (1), 2014	Number 16 VOL. 16 (2), 2013
Number 16 VOL. 16 (1), 2013	Number 15 VOL. 15 (2), 2012
Number 15 VOL. 15, 2012 Supplement	Number 15 Vol. 15 (1), 2012
Number 14 14 - Vol. 14 (2), 2011	Number 14 The 9th Balkan Congress of Medical Genetics
Number 14 14 - Vol. 14 (1), 2011	Number 13 Vol. 13 (2), 2010
Number 13 Vol.13 (1), 2010	Number 12 Vol.12 (2), 2009
Number 12 Vol.12 (1), 2009	Number 11 Vol.11 (2),2008
Number 11 Vol.11 (1),2008	Number 10 Vol.10 (2), 2007
Number 10 10 (1),2007	Number 9 1&2, 2006
Number 9 3&4, 2006	Number 8 1&2, 2005
Number 8 3&4, 2004	Number 7 1&2, 2004
Number 6 3&4, 2003	Number 6 1&2, 2003
Number 5 3&4, 2002	Number 5 1&2, 2002
Number 4 Vol.3 (4), 2000	Number 4 Vol.2 (4), 1999
Number 4 Vol.1 (4), 1998	Number 4 3&4, 2001
Number 4 1&2, 2001	Number 3 Vol.3 (3), 2000
Number 3 Vol.2 (3), 1999	Number 3 Vol.1 (3), 1998
Number 2 Vol.3(2), 2000	Number 2 Vol.1 (2), 1998
Number 2 Vol.2 (2), 1999	Number 1 Vol.3 (1), 2000
Number 1 Vol.2 (1), 1999	Number 1 Vol.1 (1), 1998

About the journal ::: Editorial ::: Subscription ::: Information for authors ::: Contact