ROLE OF THE CYP1A2 GENE POLYMORPHISM ON
EARLY AGEING FROM OCCUPATIONAL EXPOSURE
Eshkoor SA1,2,*, Ismail P1, Rahman SA2, Moin S2, Adon MY3
*Corresponding Author: Dr. Sima A. Eshkoor, Institute of Gerontology, Faculty of Medicine and Health Sciences,
Universiti Putra Malaysia (UPM), 43400, Malaysia; Tel.: +60-129-797-812; Fax: +60-389-472-744;
E-mail: simaataolahi@yahoo.com
page: 45
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Abstract
The ageing process is influenced by many internal
and external factors. The toxic substances in the
environment can cause genomic damages to cells,
which increase the risk of early ageing. Furthermore,
the cytochrome P450 1A2 (CYP1A2) gene polymorphism
is a susceptibility factor and may enhance the
risk of DNA damage in cells. The current study was
carried out to show whether occupational exposure
could cause genotoxicity in cells carrying the CYP1A2
gene polymorphism, thus enhancing the likelihood of
early ageing. This study was conducted on mechanical
workshop workers and a control group by collecting
buccal cells from their mouths. Restriction fragment
length polymorphism-polymerase chain reaction
(RFLP-PCR) was used to identify the CYP1A2 gene
polymorphism in the cells. In addition, three extra
methods including micronuclei (MN) test, comet assay
and real-time PCR (RT-PCR) were applied to
determine the effects of gene polymorphisms on DNA
damage and ageing from occupational exposure. The
results showed that DNA damage in the cells carrying
the mutated genotype was higher than the wild genotype.
In addition, the difference in MN frequency (p
= 0.001) and relative telomere length (p = 0.002) between
workers and controls was significant (p <0.05)
in the mutated genotype. The findings indicated a possible
protective effect of gene polymorphism against
early ageing, which was characterized by lack of a
significant influence of CYP1A2 gene polymorphism
on genetic material in the subjects (p >0.05). It was
concluded that the CYP1A2 gene could be a contributing
factor to prevent early ageing from occupational
exposure.
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