RAPID DETECTION OF HUMAN TORQUE TENO VIRUSES
USING HIGH-RESOLUTION MELTING ANALYSIS
Spandole S1*, Cimponeriu D1, Toma M1, Radu I1, Ion DA2
*Corresponding Author: Ms. Sonia Spandole (Ph.D. Student), Department of Genetics, University of Bucharest,
Intrarea Portocalelor Street, No 1-3, 060101, Bucharest, Romania; Tel.: 004-0764-824-281, Fax: 004-0213-181-
565; E-mail: sonia.spandole@gmail.com
page: 55
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RESULTS
The prevalence of TTV, TTMDV and TTMV in
saliva samples was 71.0, 31.0 and 54.0%, respectively.
There were no qualitative differences in the genotyping
results obtained for the same sample extracted with
four commercial kits and amplified with three different
DNA polymerases. The distribution of TTVs did not
differ in blood and saliva samples (Table 2). Ten samples with triple coinfection were selected
for the HRMA using SensiMix®, SYBR Green Master
Mix and Maxima® SYBR Green qPCR commercial kits
(Figure 1a, Figure 2 and Figure 3). Prior to HRMA, amplicons
were verified by gel electrophoresis (Figure 4).
The three major melting curve patterns corresponding
to TTV, TTMDV and TTMV can be easily
distinguished regardless of the kit used, although they
present slightly different aspects. The melting curve
patterns were similar when samples were retested
under the same conditions.
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