Carriers of chromosomal structural abnormalities such as translocations are of high importance among other IVF complication cases since they have an increased risk of producing aneuploid gamete cells, resulting in implantation failure or early abortions. Young couple underwent IVF treatment twice in our hospital. ICSI technique was performed in both cases due to husband’s oligoastenoteratospermic sperm. Although fertilization was successful, after transfer of embryos no pregnancy was achieved. Because of the implantation failure karyotype analysis was performed in both patients. We found husband’s karyotype to be normal, while wife’s karyotype showed balanced autosomal reciprocal whole arm translocation 46 XX t(3,5). The same translocation was later found in women’s father who had 3 phenotypically normal children.