Background: Recurrent spontaneous abortion, defined as at least three consecutive pregnancy loss, occurs in 10% of all pregnancies. In the etiology of recurrent spontaneous abortion has been reported wide variety abnormalities such as anatomic, endocrinologic, genetic abnormalities. In this study, we aimed to share karyotyping results of couples with recurrent spontaneous abortions that may be contributed to elucidate the genetic basis of this condition and warn the physicians about the cytogenetic abnormalities in cases with recurrent spontaneous abortions. Methods: We reviewed the cytogenetic results of the patients with at least two abortions, referred to Firat (Euphrates) University, College of Medicine, Department of Medical Biology and Genetics in 6-year period between 2000-2005, retrospectively. Results: We report a total of 421 couples (842 patients). 22 males (2.61%) and 8 (0.95 %) females had abnormal karyotypes. The total rate of abnormalities was 3.56%. Conclusion: All these data show that cytogenetic evaluation is necessery for an accurate approach to elucidate the causes and the physicians should also be careful for diversity of the chromosomal abnormalities that play important roles in the etiology of recurrent spontaneous abortions.