THE RELATIONSHIP BETWEEN TRANSCRIPT EXPRESSION
LEVELS OF NUCLEAR ENCODED (TFAM, NRF1) AND
MITOCHONDRIAL ENCODED (MT-CO1) GENES IN SINGLE
HUMAN OOCYTES DURING OOCYTE MATURATION
Ghaffari Novin M, Allahveisi A, Noruzinia M, Farhadifar F,
Yousefian E, Dehghani Fard A, Salimi M
*Corresponding Author: Dr. Azra Allahveisi, Department of Anatomical Sciences, Faculty of Medicine, Kurdistan
University of Medical Sciences, Pasdaran Street, Sanandaj, Iran. Tel: +98-873-664-673. Fax: +98-873-364-
674. E-mail: allavaisie@gmail.com
page: 39
download article in pdf format
|
|
Abstract
In some cases of infertility in women, human
oocytes fail to mature when they reach the metaphase
II (MII) stage. Mitochondria plays an important role
in oocyte maturation. A large number of mitochondrial
DNA (mtDNA), copied in oocytes, is essential
for providing adenosine triphosphate (ATP) during
oocyte maturation. The purpose of this study was
to identify the relationship between transcript expression
levels of the mitochondrial encoded gene
(MT-CO1) and two nuclear encoded genes, nuclear
respiratory factor 1 (NRF1) and mitochondrial transcription
factor A (TFAM) in various stages of human
oocyte maturation. Nine consenting patients,
age 21-35 years old, with male factors were selected
for ovarian stimulation and intracytoplasmic sperm
injection (ICSI) procedures. mRNA levels of mitochondrial-
related genes were performed by singlecell
TaqManŽ quantitative real-time polymerase
chain reaction (qRT-PCR). There was no significant
relationship between the relative expression levels
in germinal vesicle (GV) stage oocytes (p = 0.62).
On the contrary, a significant relationship was seen
between the relative expression levels of TFAM and
NRF1 and the MT-CO1 genes at the stages of metaphase
I (MI) and MII (p = 0.03 and p = 0.002). A
relationship exists between the transcript expression
levels of TFAM and NRF1, and MT-CO1 genes in
various stages of human oocyte maturation.
|
|
|
|
 |
Number 27
VOL. 27 (2), 2024 |
Number 27
VOL. 27 (1), 2024 |
Number 26
Number 26 VOL. 26(2), 2023 All in one |
Number 26
VOL. 26(2), 2023 |
Number 26
VOL. 26, 2023 Supplement |
Number 26
VOL. 26(1), 2023 |
Number 25
VOL. 25(2), 2022 |
Number 25
VOL. 25 (1), 2022 |
Number 24
VOL. 24(2), 2021 |
Number 24
VOL. 24(1), 2021 |
Number 23
VOL. 23(2), 2020 |
Number 22
VOL. 22(2), 2019 |
Number 22
VOL. 22(1), 2019 |
Number 22
VOL. 22, 2019 Supplement |
Number 21
VOL. 21(2), 2018 |
Number 21
VOL. 21 (1), 2018 |
Number 21
VOL. 21, 2018 Supplement |
Number 20
VOL. 20 (2), 2017 |
Number 20
VOL. 20 (1), 2017 |
Number 19
VOL. 19 (2), 2016 |
Number 19
VOL. 19 (1), 2016 |
Number 18
VOL. 18 (2), 2015 |
Number 18
VOL. 18 (1), 2015 |
Number 17
VOL. 17 (2), 2014 |
Number 17
VOL. 17 (1), 2014 |
Number 16
VOL. 16 (2), 2013 |
Number 16
VOL. 16 (1), 2013 |
Number 15
VOL. 15 (2), 2012 |
Number 15
VOL. 15, 2012 Supplement |
Number 15
Vol. 15 (1), 2012 |
Number 14
14 - Vol. 14 (2), 2011 |
Number 14
The 9th Balkan Congress of Medical Genetics |
Number 14
14 - Vol. 14 (1), 2011 |
Number 13
Vol. 13 (2), 2010 |
Number 13
Vol.13 (1), 2010 |
Number 12
Vol.12 (2), 2009 |
Number 12
Vol.12 (1), 2009 |
Number 11
Vol.11 (2),2008 |
Number 11
Vol.11 (1),2008 |
Number 10
Vol.10 (2), 2007 |
Number 10
10 (1),2007 |
Number 9
1&2, 2006 |
Number 9
3&4, 2006 |
Number 8
1&2, 2005 |
Number 8
3&4, 2004 |
Number 7
1&2, 2004 |
Number 6
3&4, 2003 |
Number 6
1&2, 2003 |
Number 5
3&4, 2002 |
Number 5
1&2, 2002 |
Number 4
Vol.3 (4), 2000 |
Number 4
Vol.2 (4), 1999 |
Number 4
Vol.1 (4), 1998 |
Number 4
3&4, 2001 |
Number 4
1&2, 2001 |
Number 3
Vol.3 (3), 2000 |
Number 3
Vol.2 (3), 1999 |
Number 3
Vol.1 (3), 1998 |
Number 2
Vol.3(2), 2000 |
Number 2
Vol.1 (2), 1998 |
Number 2
Vol.2 (2), 1999 |
Number 1
Vol.3 (1), 2000 |
Number 1
Vol.2 (1), 1999 |
Number 1
Vol.1 (1), 1998 |
|
|
