Objective: The secretion of type 1 IFN in response to herpes simplex virus type 1 (HSV-1) and 2  in vitro is mediated by TLR9. In this study, we purpose whether the any association between TLR9 1635G/A poymorphisms and HSV1,2,6,7 positivity and the incidence of HSV1,2,6,7 are positively linked to infection in the prostate tissue samples.

Material and methods:  The DNA was extracted from the parraffin embedded tissues. Four different HSV DNA detection were done by polymerase chain reaction (PCR). Genotyping of TLR9 1635 G/A polymorphism was performed by PCR-Restriction Fragment Lenght Polymorphism (PCR-RFLP).  Fischer's test was used for statistical analysis.

Results: HSV DNA positive was detected in 12 of 21 (57.1%) prostate cancer (PCa), in 15 of 24 (62.5%)  prostatic intraepithelial neoplasia (PIN) and in 14 of 21 (66.7%) benign prostatic hyperplasia (BPH), all of total 66 samples. Differences in TLR9 1635A/G allelic frequencies were not statistically observed, neither between PCa, PIN and BPH nor between HSV-positive and HSV-negative all samples.

Discussion: Our findings indicate that it was not found statiscially significant association between  HSV1,2,6,7 and TLR9 1635 G/A polymorphism. As result, we consider that future investigations are needed to provided conclusive evidence on the role of these pathogen and gene in the prostate cancer.